Effect of Alcohol Structure on the Optimum Condition for Novozym 435-Catalyzed Synthesis of Adipate Esters

Table 4

Optimum conditions for lipase-catalyzed synthesis of adipate esters.

Alcohol	Temperature	Time	Enzyme	Substrate	Predicted	Actual
	(^∘C)	(min)	amount (mg)	molar ratio	yield (%)	yield (%)
n-butanol	56	268	35	7.7	100.0	97.2
iso-butanol	54	220	20	6.0	100.0	97.0
sec-butanol	55	264	35	6.4	100.0	96.3
tert-butanol	65	30	350	3.8	42.8	39.1