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Biotechnology Research International
Volume 2011 (2011), Article ID 478925, 4 pages
Research Article

Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity

1ITMC, RWTH Aachen University, Worringerweg 1, 52056 Aachen, Germany
2Insititute of Chemistry, Technical University of Berlin, Straβe des 17. Juni 124, 10623 Berlin, Germany
3DECHEMA e.V. Karl-Winnacker-Institut, Theodor-Heuss-Allee 25, 60486 Frankfurt am Main, Germany

Received 23 March 2011; Revised 25 May 2011; Accepted 8 June 2011

Academic Editor: Manuel Canovas

Copyright © 2011 Dessy Natalia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Benzaldehyde lyase from Pseudomonas fluorescens Biovar I. (BAL, EC is a versatile catalyst for the organic synthesis of chiral α-hydroxy ketones. To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable. Here, a new robust and easy-to-handle assay based on UV absorption is presented. The assay developed is based on the ligation of the α-hydroxy ketone (R)-2,2′-furoin from 2-furaldehyde. A robust assay with direct monitoring of the product is facilitated with a convenient concentration working range minimising experimental associated with low concentrations.