Enhancement of Bacillus subtilis Lipopeptide Biosurfactants Production through Optimization of Medium Composition and Adequate Control of Aeration

Table 1

Effect of the variation of glucose concentrations on biomass and biosurfactant production by Bacillus subtilis SPB1 strain.

Glucose (g/L)	Biosurfactants (mg/L)	CFU (10^7 CFU/mL)	Sugar utilisation (%)	*Relative biosurfactants yield (mg/10^10 CFU)
15	420±5	18±1	100	23.33
20	480±8	20±2	100	24.00
25	540±10	22±1	100	24.54
30	600±15	24±2	100	25.00
35	660±5	27±2	100	24.44
40	720±7	28±1	99	25.71
45	690±6	30±3	97	23.00

*Relative biosurfactant yield was calculated as the ratio of biosurfactant (mg/L) over CFU (10¹⁰  CFU/L), both of them were determined at the end of fermentation.