Enhancement of Bacillus subtilis Lipopeptide Biosurfactants Production through Optimization of Medium Composition and Adequate Control of Aeration

Table 3

Aeration effect on SPB1 biosurfactants production into 2,6 L fermentor using the formulated medium.

Aeration profile (%)	Biosurfactants (mg/L)	CFU (10^8 cells/mL)	*Relative biosurfactants yield (mg/10^10cells)
10	2410.160±23	15.30±2	15.75
20	3801.120±34	16.60±3	22.89
30	4922.540±25	20.00±1	24.61
40	4230.423±13	27.50±3	15.38
60	2609.140±11	36.00±1	7.24

*Relative biosurfactant yield was calculated as the ratio of biosurfactant (mg/L) over CFU (10¹⁰ CFU/L), both of them were determined at the end of fermentation.