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Biotechnology Research International
Volume 2011 (2011), Article ID 708043, 7 pages
Research Article

Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs

1Department of Chemical Engineering, Annamalai University, Annamalainagar, Tamilnadu 608002, India
2Department of Chemical Engineering, Sohar University, Sohar 311, Oman

Received 20 December 2010; Accepted 28 January 2011

Academic Editor: Triantafyllos Roukas

Copyright © 2011 M. Dilipkumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH4NO3, MnSO4⋅7H2O, Soya bean cake, and K2HPO4 were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH4NO3—6.63 mg/gds, MnSO4⋅7H2O—26.16 mg/gds, Soya bean cake—60.6 mg/gds, and K2HPO4—5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds.