Research Article
Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs
Table 4
Central composite design (CCD) of factors in coded levels with enzyme activity as response.
| Run no. | | | | | Inulinase activity (U/g dry substrate) | Experimental | Predicted |
| 1 | −1 | 1 | −1 | −1 | 32.25 | 29.92 | 2 | 1 | 1 | 1 | 1 | 19.38 | 24.17 | 3 | 1 | 1 | −1 | −1 | 25.78 | 25.83 | 4 | 0 | 0 | −2 | 0 | 41.94 | 35.42 | 5 | −1 | 1 | −1 | 1 | 40.83 | 47.50 | 6 | 0 | 0 | 0 | 0 | 58.43 | 59.43 | 7 | 0 | 0 | 0 | 0 | 60.75 | 59.43 | 8 | −1 | −1 | −1 | 1 | 54.69 | 55.25 | 9 | 0 | 0 | 0 | 0 | 59.23 | 59.43 | 10 | 1 | 1 | −1 | 1 | 15.32 | 12.92 | 11 | 2 | 0 | 0 | 0 | 23.85 | 26.08 | 12 | 1 | −1 | 1 | −1 | 44.89 | 47.83 | 13 | −1 | −1 | −1 | −1 | 23.92 | 28.17 | 14 | 1 | −1 | −1 | 1 | 14.37 | 24.67 | 15 | 1 | −1 | 1 | 1 | 50.03 | 42.92 | 16 | −2 | 0 | 0 | 0 | 45.18 | 42.75 | 17 | −1 | 1 | 1 | −1 | 25.30 | 24.67 | 18 | 0 | 0 | 0 | 0 | 60.34 | 59.43 | 19 | −1 | −1 | 1 | 1 | 45.59 | 55.50 | 20 | 0 | 2 | 0 | 0 | 19.42 | 23.42 | 21 | −1 | 1 | 1 | 1 | 45.69 | 40.75 | 22 | −1 | −1 | 1 | −1 | 36.80 | 29.92 | 23 | 1 | −1 | −1 | −1 | 31.86 | 28.08 | 24 | 0 | 0 | 0 | 2 | 46.05 | 37.25 | 25 | 1 | 1 | 1 | −1 | 48.29 | 38.58 | 26 | 0 | 0 | 0 | 0 | 57.44 | 59.43 | 27 | 0 | −2 | 0 | 0 | 45.30 | 40.42 | 28 | 0 | 0 | 0 | 0 | 60.23 | 59.43 | 29 | 0 | 0 | 2 | 0 | 42.40 | 48.42 | 30 | 0 | 0 | 0 | −2 | 16.42 | 24.58 |
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