Applicability of Three Alternative Instruments for Food Authenticity Analysis:GMO Identification

Table 1

Amplicon sizes associated with the “GMO Multiplex Screen” protocol. Amplicons sizes are shown in bp. “Theoretical size (bp)” refers to the calculated number of base pairs in the amplicon based on available DNA sequence information. The final three columns refer to the average bp sizes of the amplicons as determined by the Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MultiNA, for the positive control used in this study. Note that the NOS terminator and CaMV 35s promoter PCR products were seen as one peak on all three instruments.

Primer set	Theoretical size (bp)	Agilent Bioanalyzer 2100 sizes (bp)	Lab901 TapeStation sizes (bp)	Shimadzu MultiNA sizes (bp)
Zein	68	80	71	77
Lectin	80	90	81	86
CaMV	104	108	104	108
NOS terminator	118	131	120	127
CaMV 35S promoter	123