Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces
Figure 4
Western blot analysis of (a) HSP90 and (b) α-SM actin. Blots represent 5 μg of total protein from cell lysates, with each series of blots repeated three times. Histograms show the volume of bands expressed as a percentage of the maximum band in the presence of a uniform threshold applied to each series of blots. (PDADMA/PSS)2 consistently generated the maximum threshold for HSP90 blots, and (PFPVP/Nafion)2 consistently generated the maximum threshold when blots were probed specifically for α-SM actin (exclusive of other isoforms). Significant variation was seen in the expression of HSP90 when (PDADMA/PSS)2 was compared to uncoated glass, and in the expression of α-SM actin when (PFPVP/Nafion)2 was compared to (PDADMA/PSS)2. Values are expressed as the mean ± S.E., with variations in the means compared by 1-way ANOVA. *, **.
