Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces
Figure 6
Fluorescent double staining for multiple actin isoforms (red) and α-SM actin (green) on (a) uncoated glass, (c) (PDADMA/PSS)2, (e) (PFPVP/Nafion)2, and (g) (PAH/PAA-co-PAEDAPS)2. Positive signals were detected with Alexa Fluor 488 (green) and Alexa Fluor 546 (red) secondary antibodies. For double-stained images, nuclei are stained blue with DAPI. For enhanced clarity of the observed trends in α-SM actin patterns of expression, fluorescent staining exclusive for α-SM actin (red) in the absence of DAPI is shown on (b) uncoated glass, (d) (PDADMA/PSS)2, (f) (PFPVP/Nafion)2, and (h) (PAH/PAA-co-PAEDAPS)2, utilizing the Alexa Fluor 546 secondary antibody.
