Table of Contents Author Guidelines Submit a Manuscript
Biotechnology Research International
Volume 2011 (2011), Article ID 897931, 7 pages
http://dx.doi.org/10.4061/2011/897931
Research Article

Involvement of Physical Parameters in Medium Improvement for Tannase Production by Aspergillus niger FETL FT3 in Submerged Fermentation

1School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
2School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia

Received 29 April 2011; Accepted 13 June 2011

Academic Editor: Yu Hong Wei

Copyright © 2011 I. Darah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aspergillus niger FETL FT3, a local extracellular tannase producer strain that was isolated from one of dumping sites of tannin-rich barks of Rhizophora apiculata in Perak, Malaysia. This fungus was cultivated in 250 mL Erlenmeyer flask under submerged fermentation system. Various physical parameters were studied in order to maximize the tannase production. Maximal yield of tannase production, that is, 2.81 U per mL was obtained on the fourth day of cultivation when the submerged fermentation was carried out using liquid Czapek-Dox medium containing (percent; weight per volume) 0.25% NaNO3, 0.1% KH2PO4, 0.05% MgSO4 ·7H2O, 0.05% KCl, and 1.0% tannic acid. The physical parameters used initial medium pH of 6.0, incubation temperature of 3 0 C , agitation speed of 200 rpm and inoculums size of 6 × 1 0 6  spores/ ml. This research has showed that physical parameters were influenced the tannase production by the fungus with 156.4 percent increment.