Generation of HIV-1 and Internal Control Transcripts as Standards for an In-House Quantitative Competitive RT-PCR Assay to Determine HIV-1 Viral Load

Table 4

Determination of the IC transcript (T-IC-2^b) working dilution in a qRT-PCR assay.

T-IC-2^b dilution	𝑟^2	𝑏	Accuracy IS 10^3 IU/mL (Log10 VLt-Log10 VLe)	Accuracy IS 10^4 IU/mL (Log10 VLt-Log10 VLe)
1 : 4 × 10^4	0.14	−0.02	26.62	65.78
1 : 4 × 10^5	0.87	0.31	−1.87	−3.05
1 : 4 × 10^6	0.95	0.45	0.4	−0.45
1 : 4 × 10^7	0.86	0.33	2.01	0.92

𝑟²: determination coefficient; 𝑏: curve slope; IS: HIV-1 RNA international standard (code 97/656); VLt: theoretical viral load; VLe: estimated viral load.