Table 4: Determination of the IC transcript (T-IC-2b) working dilution in a qRT-PCR assay.

T-IC-2b dilution Accuracy IS 103 IU/mL (Log10 VLt-Log10 VLe)Accuracy IS 104 IU/mL (Log10 VLt-Log10 VLe)

1 : 4 × 1040.14−0.0226.6265.78
1 : 4 × 1050.870.31−1.87−3.05
1 : 4 × 1060.950.450.4−0.45
1 : 4 × 1070.860.332.010.92

: determination coefficient; : curve slope; IS: HIV-1 RNA international standard (code 97/656); VLt: theoretical viral load; VLe: estimated viral load.