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Biotechnology Research International
Volume 2011 (2011), Article ID 967925, 11 pages
http://dx.doi.org/10.4061/2011/967925
Research Article

Bioremediation and Detoxification of Synthetic Wastewater Containing Triarylmethane Dyes by Aeromonas hydrophila Isolated from Industrial Effluent

1School of Biology, Aristotle University of Thessaloniki, Thessaloniki 54124, Macedonia, Greece
2Department of Microbiology, University of Port Harcourt, Port Harcourt 500004, Nigeria

Received 3 February 2011; Revised 23 May 2011; Accepted 24 May 2011

Academic Editor: Yu Hong Wei

Copyright © 2011 Chimezie Jason Ogugbue and Thomas Sawidis. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Economical and bio-friendly approaches are needed to remediate dye-contaminated wastewater from various industries. In this study, a novel bacterial strain capable of decolorizing triarylmethane dyes was isolated from a textile wastewater treatment plant in Greece. The bacterial isolate was identified as Aeromonas hydrophila and was shown to decolorize three triarylmethane dyes tested within 24 h with color removal in the range of 72% to 96%. Decolorization efficiency of the bacterium was a function of operational parameters (aeration, dye concentration, temperature, and pH) and the optimal operational conditions obtained for decolorization of the dyes were: pH 7-8, 35C and culture agitation. Effective color removal within 24 h was obtained at a maximum dye concentration of 50 mg/L. Dye decolorization was monitored using a scanning UV/visible spectrophotometer which indicated that decolorization was due to the degradation of dyes into non-colored intermediates. Phytotoxicity studies carried out using Triticum aestivum, Hordeum vulgare, and Lens esculenta revealed the triarylmethane dyes exerted toxic effects on plant growth parameters monitored. However, significant reduction in toxicity was obtained with the decolorized dye metabolites thus, indicating the detoxification of the dyes following degradation by Aeromonas hydrophila.