Biotechnology Research International

Research Article

In Vitro Propagation of Muña-Muña (Clinopodium odorum (Griseb.) Harley)

Table 3

Effect of nutrient media and PGRs (mg ⁻¹) concentrations on in vitro C.odorum. Values followed by the same letter within a column are not significantly different at the , according ANOVA analysis and by Duncan’s test.


Culture media	BAP	NAA	Principal shoot length (cm)	Number of nodes (principal shoot)	Number of axillaries shoots

1/2 MS-0	0.00	0.00	6.06 ± 0.71^a	9.46 ± 0.91^a	1.00 ± 0.35^a
1/2 MS-1	0.50	0.50	8.91 ± 0.68^a	11.14 ± 0.88^a	0.79 ± 0.34^a
1/2 MS-2	0.50	1.00	9.81 ± 0.68^a	11.93 ± 0.88^a	0.79 ± 0.34^a
1/2 MS-3	0.50	1.50	7.13 ± 0.81^a	7.89 ± 1.09^a	0.44 ± 0.43^a
1/2 MS-4	1.00	0.50	3.91 ± 0.96^a	5.14 ± 1.24^a	1.00 ± 0.48^a
1/2 MS-5	1.00	1.00	4.69 ± 0.90^a	7.89 ± 1.09^a	1.22 ± 0.43^a
1/2 MS-6	1.00	1.50	12.14 ± 0.64^b	16.38 ± 0.82^b	1.75 ± 0.32^a
1/2 MS-7	1.50	0.50	2.70 ± 1.04^a	4.17 ± 1.34^a	1.50 ± 0.52^a
1/2 MS-8	1.50	1.00	5.22 ± 0.81^a	7.50 ± 1.04^a	3.60 ± 0.40^a
1/2 MS-9	1.50	1.50	—	—	—

SH-0	0.00	0.00	7.46 ± 1.14^a	6.60 ± 1.47^a	1.80 ± 0.57^a
SH-1	0.50	0.50	8.89 ± 0.96^a	10.14 ± 1.24^b	1.29 ± 0.48^a
SH-2	0.50	1.00	8.60 ± 1.47^a	10.00 ± 1.89^a	1.67 ± 0.74^a
SH-3	0.50	1.50	4.15 ± 1.80^a	4.50 ± 1.32^a	1.00 ± 0.90^a
SH-4	1.00	0.50	3.56 ± 1.14^a	4.40 ± 1.47^a	0.80 ± 0.57^a
SH-5	1.00	1.00	6.84 ± 0.96^a	7.71 ± 1.24^a	0.71 ± 0.48^a
SH-6	1.00	1.50	5.85 ± 1.04^a	8.33 ± 1.34^a	1.17 ± 0.52^a
SH-7	1.50	0.50	4.38 ± 1.27^a	6.25 ± 1.64^a	1.03 ± 0.48^a
SH-8	1.50	1.00	—	—	—
SH-9	1.50	1.50	6.20 ± 1.47^a	8.33 ± 1.89^a	4.67 ± 0.74^a

WP-0	0.00	0.00	7.74 ± 0.68^a	7.71 ± 0.88^a	0.93 ± 0.34^a
WP-1	0.50	0.50	11.24 ± 0.77^a	12.91 ± 0.99^a	1.27 ± 0.39^a
WP-2	0.50	1.00	12.67 ± 0.66^b	13.07 ± 0.85^a	1.53 ± 0.33^a
WP-3	0.50	1.50	12.61 ± 0.74^b	14.42 ± 0.95^a	1.17 ± 0.37^a
WP-4	1.00	0.50	9.39 ± 0.96^a	9.71 ± 1.24^a	0.29 ± 0.42^a
WP-5	1.00	1.00	10.19 ± 0.90^a	10.38 ± 1.16^a	1.25 ± 0.45^a
WP-6	1.00	1.50	9.14 ± 0.81^a	10.50 ± 1.04^a	0.80 ± 0.40^a
WP-7	1.50	0.50	5.74 ± 0.71^a	8.85 ± 0.91^a	0.69 ± 0.35^a
WP-8	1.50	1.00	2.57 ± 1.47^a	6.33 ± 1.89^a	0.33 ± 0.74^a
WP-9	1.50	1.50	2.55 ± 1.27^a	6.25 ± 1.64^a	0.75 ± 0.64^a

1/2 B5-0	0.00	0.00	2.37 ± 1.44^a	4.33 ± 1.77^a	0.67 ± 1.00^a
1/2 B5-1	0.50	0.50	3.70 ± 0.83^a	4.33 ± 1.02^a	5.33 ± 0.58^a
1/2 B5-2	0.50	1.00	6.21 ± 0.88^a	6.63 ± 1.08^a	6.63 ± 0.61^a
1/2 B5-3	0.50	1.50	4.93 ± 0.83^a	6.78 ± 1.02^a	7.00 ± 0.58^b
1/2 B5-4	1.00	0.50	4.72 ± 1.25^a	7.00 ± 1.53^a	6.75 ± 0.87^a
1/2 B5-5	1.00	1.00	—	—	—
1/2 B5-6	1.00	1.50	6.12 ± 1.02^a	6.83 ± 1.25^a	3.00 ± 0.71^a
1/2 B5-7	1.50	0.50	2.39 ± 0.94^a	3.71 ± 1.16^a	4.14 ± 0.65^a
1/2 B5-8	1.50	1.00	1.90 ± 2.49^a	5.00 ± 3.06^b	—
1/2 B5-9	1.50	1.50	4.03 ± 1.02^a	4.33 ± 1.25^a	3.00 ± 0.71^a