Research Article

DNA Damage Protecting Activity and Free Radical Scavenging Activity of Anthocyanins from Red Sorghum (Sorghum bicolor) Bran

Table 2

Concentration dependent free radical scavenging activity of methanol and methanol extracts of anthocyanin from red sorghum bran.

Antioxidant assaysStandards (%)Methanol extract (%)Acidified methanol extract (%)
BHTAscorbic acid1 μg10 μg50 μg100 μg1 μg10 μg50 μg100 μg

DPPH assay95.896.694.796.396.797.595.895.89697.8
Superoxide radical scavenging assay60.469.97.62936.542.511.237.54553.8
Hydroxyl radical scavenging assay99.869.930.484.598.598.87389.798.699
Reducing power (Absorbance)0.1380.5770.0240.1840.5520.8400.0140.0370.4450.984
Metal chelating79834674.480.385.148.375.283.785.2
Hydrogen peroxide94.640.669.585.390.247.988.690.491.3
Deoxyribose degradation site-specific assay99.148.48589.691.391.87288.991.791.8
Deoxyribose degradation non-site-specific assay80.233.974.879.881.986.659.87981.482
Anti FeCl3–H2O2 stimulated Linoleic acid77.287.882.283.585.586.273.18184.392