Research Article
DNA Damage Protecting Activity and Free Radical Scavenging Activity of Anthocyanins from Red Sorghum (Sorghum bicolor) Bran
Table 2
Concentration dependent free radical scavenging activity of methanol and methanol extracts of anthocyanin from red sorghum bran.
| Antioxidant assays | Standards (%) | Methanol extract (%) | Acidified methanol extract (%) | BHT | Ascorbic acid | 1 μg | 10 μg | 50 μg | 100 μg | 1 μg | 10 μg | 50 μg | 100 μg |
| DPPH assay | 95.8 | 96.6 | 94.7 | 96.3 | 96.7 | 97.5 | 95.8 | 95.8 | 96 | 97.8 | Superoxide radical scavenging assay | 60.4 | 69.9 | 7.6 | 29 | 36.5 | 42.5 | 11.2 | 37.5 | 45 | 53.8 | Hydroxyl radical scavenging assay | 99.8 | 69.9 | 30.4 | 84.5 | 98.5 | 98.8 | 73 | 89.7 | 98.6 | 99 | Reducing power (Absorbance) | 0.138 | 0.577 | 0.024 | 0.184 | 0.552 | 0.840 | 0.014 | 0.037 | 0.445 | 0.984 | Metal chelating | 79 | 83 | 46 | 74.4 | 80.3 | 85.1 | 48.3 | 75.2 | 83.7 | 85.2 | Hydrogen peroxide | 94.6 | — | 40.6 | 69.5 | 85.3 | 90.2 | 47.9 | 88.6 | 90.4 | 91.3 | Deoxyribose degradation site-specific assay | 99.1 | 48.4 | 85 | 89.6 | 91.3 | 91.8 | 72 | 88.9 | 91.7 | 91.8 | Deoxyribose degradation non-site-specific assay | 80.2 | 33.9 | 74.8 | 79.8 | 81.9 | 86.6 | 59.8 | 79 | 81.4 | 82 | Anti FeCl3–H2O2 stimulated Linoleic acid | 77.2 | 87.8 | 82.2 | 83.5 | 85.5 | 86.2 | 73.1 | 81 | 84.3 | 92 |
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