Research Article

High-Frequency Regeneration of the Drought-Tolerant Tree Melia volkensii Gurke Using Low-Cost Agrochemical Thidiazuron

Figure 2

(a) Well-defined multiple microshoots formed from the embryogenic callus 27 days after transfer of callus to B5 + 0 PGR medium; cumulative age 48 days. (b) An elongated phenotypically normal M. volkensii shoot after 25 days on 1/2 MS medium supplemented with 0.1 mg/L BAP and 10% coconut water, following initial elongation for 27 days on 1/2 MS media supplemented with 0.1 mg/L BAP only. Cumulative age from callus induction = 104 days.
818472.fig.002a
(a)
818472.fig.002b
(b)