Table of Contents
Biotechnology Research International
Volume 2014, Article ID 127848, 6 pages
http://dx.doi.org/10.1155/2014/127848
Research Article

Expeditious Quantification of Lignocellulolytic Enzymes from Indigenous Wood Rot and Litter Degrading Fungi from Tropical Dry Evergreen Forests of Tamil Nadu

1Mycology Laboratory, Centre for Advanced Studies in Botany, University of Madras, Chennai 600 025, India
2Centre for Nanoscience and Technology, Anna University, Chennai 600 025, India

Received 6 November 2013; Accepted 31 December 2013; Published 26 February 2014

Academic Editor: Triantafyllos Roukas

Copyright © 2014 Jenefar Sudarson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this study thirty wood rotting and litter degrading basidiomycetes were screened for the production of lignocellulolytic enzymes such as, laccase, peroxidase, and cellulase using rapid micro quantification assay. Out of the 30 indigenous isolates Trametes gibbosa was identified to be a potential lignocellulolytic enzyme producer, producing a maximum amount of cellulase (  IU/L) and laccase (  U/L). Moreover, it is the second leading producer of peroxidase enzyme (  U/L). Tricholomopsis sp. a wood rot basidiomycete was found to be the leading lignin decomposer with maximum peroxidase activity (  U/L) and second maximum laccase activity (  U/L). However, its cellulolytic potential was found to be moderate (  U/L). A higher level of lignocellulolytic enzymes was recorded in wood rotting basidiomycetes, whereas very low levels of lignolytic enzymes were found in litter inhabiting basidiomycetes. However, their cellulolytic potential was found to be moderate.