Biotechnology Research International

Research Article

Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3

Table 2

(a) Experimental ranges and levels of the independent variable used in RSM. (b) Central composite design matrix for the experimental design and respective response for protease activity.

(a)


Variable	Experimental ranges and levels
Variable	−2.378	−1	0	+1	+2.378

Tryptone	0.24	3.00	5.00	7.00	9.76
Soya peptone	3.81	4.50	5.00	5.50	6.19
Dextrose	0.31	1.00	1.50	2.00	2.69
NaCl	3.62	5.00	6.00	7.00	8.38
Dipotassium phosphate	0.41	0.75	1.00	1.25	1.59

(b)


Std. order	Experimental coded values of different variables					Protease activity (U/mL)
Std. order						Actual	Predicted

1	−1	−1	−1	−1	−1		1187.04
2	1	−1	−1	−1	−1		1327.02
3	−1	1	−1	−1	−1		1329.21
4	1	1	−1	−1	−1		1221.26
5	−1	−1	1	−1	−1		1128.77
6	1	−1	1	−1	−1		1390.44
7	−1	1	1	−1	−1		1167.55
8	1	1	1	−1	−1		1181.28
9	−1	−1	−1	1	−1		1321.81
10	1	−1	−1	1	−1		1432.92
11	−1	1	−1	1	−1		1488.54
12	1	1	−1	1	−1		1351.72
13	−1	−1	1	1	−1		1152.72
14	1	−1	1	1	−1		1385.53
15	−1	1	1	1	−1		1216.07
16	1	1	1	1	−1		1200.94
17	−1	−1	−1	−1	1		1173.58
18	1	−1	−1	−1	1		1272.75
19	−1	1	−1	−1	1		1342.36
20	1	1	−1	−1	1		1193.59
21	−1	−1	1	−1	1		1260.05
22	1	−1	1	−1	1		1480.9
23	−1	1	1	−1	1		1325.44
24	1	1	1	−1	1		1298.36
25	−1	−1	−1	1	1		1178.03
26	1	−1	−1	1	1		1248.34
27	−1	1	−1	1	1		1371.38
28	1	1	−1	1	1		1193.75
29	−1	−1	1	1	1		1153.68
30	1	−1	1	1	1		1345.68
31	−1	1	1	1	1		1243.64
32	1	1	1	1	1		1187.7
33	−2.378	0	0	0	0		1289.12
34	2.378	0	0	0	0		1389.05
35	0	−2.378	0	0	0		1267.98
36	0	2.378	0	0	0		1249.18
37	0	0	−2.378	0	0		1335.33
38	0	0	2.378	0	0		1258.84
39	0	0	0	−2.378	0		1138.25
40	0	0	0	2.378	0		1166.92
41	0	0	0	0	−2.378		1141.46
42	0	0	0	0	2.378		1109.71
43	0	0	0	0	0		1569.75
44	0	0	0	0	0		1569.75
45	0	0	0	0	0		1569.75
46	0	0	0	0	0		1569.75
47	0	0	0	0	0		1569.75
48	0	0	0	0	0		1569.75
49	0	0	0	0	0		1569.75
50	0	0	0	0	0		1569.75