Antidepressant Fluoxetine Modulates the In Vitro Inhibitory Activity of Buffalo Brain Cystatin: A Thermodynamic Study Using UV and Fluorescence Techniques

Table 2

Inhibitory activity of brain cystatin in the presence of fluoxetine. The table shows changes in the inhibitory activity of brain cystatin after incubation for 30 min with increasing concentration of fluoxetine.  BC  (1 μM) treated with varying concentrations of fluoxetine (0.5 μM–2 μM) for 30 min in the final reaction volume of 1 mL in 0.05 M sodium phosphate buffer pH 7.5.

S. number	Fluoxetine; concentration	% Inhibitory activity; remaining
1	Cystatin alone	100
2	Cystatin + 0.5 μM fluoxetine	52 ± 0.394
3	Cystatin + 1 μM fluoxetine	48 ± 0.770
4	Cystatin + 1.5 μM fluoxetine	25 ± 0.911
5	Cystatin + 2 μM fluoxetine	10 ± 0.518

All data are expressed as mean ± S.E  for four different sets of experiments; statistical significance was conducted employing oneway ANOVA.   A probability level of 0.05 was selected showing results are significant.