Evaluation of Novel Design Strategies for Developing Zinc Finger Nucleases Tools for Treating Human Diseases

Table 5

(a) Visible band shifts with value ranging from 4570 to 500 counts: 1–26 (40%). (b) Less/no visible band shifts with value <500 counts: 27–64 (38, 60%).

(a)

9-mers	#P32	#loc	9-mers	#P32	#loc	9-mers	#P32	#loc	9-mers	#P32	#loc	9-mers	#P32	#loc
(1)CGG	4570	16697	(2) GCG	3450	22741	(4) GGC	2850	16087	(8) CCC	1900	6153	(19) GTC	950	6246
			(3) CGT	3125	6508	(5) TTG	2320	28372	(9) TAG	1850	14922	(20) GCT	800	42489
						(6) TGG	2300	55636	(10) CCG	1850	8662	(21) TAT	660	7319
						(7) GAG	2180	58721	(11) AGG	1750	41257	(22) GTA	630	15656
									(12) GGT	1440	38721	(23) GAT	560	23908
									(13) GAC	1215	5109	(24) GCA	555	40076
									(14) GTG	1200	69427	(25) AAT	550	17749
									(15) GGA	1175	50364	(26) CGC	500	4098
									(16) AGT	1150	26799
									(17) TAA	1100	9037
									(18) CAG	1010	34437

(b)

9-mers	#P32	#loc	9-mers	#P32	#loc	9-mers	#P32	#loc
(27) TCT	475	16364	(40) CGA	225	5420	(53) ACA	63	26050
(28) TCG	450	7161	(41) ATG	188	28958	(54) ACG	62	6423
(29) TAC	380	1172	(42) AAG	188	36175	(55) CAC	50	3769
(30) CCT	300	27004	(43) ACT	187	16928	(56) ATT	50	11132
(31) CAA	300	15503	(44) CTG	150	38542	(57) CTA	40	9049
(32) GTT	280	24314	(45) ATA	124	7850	(58) ATC	30	1919
(33) GGG	260	18460	(46) AGA	123	38448	(59) CTT	20	15023
(34) CAT	250	14458	(47) TTA	110	10717	(60) GAA	10	36693
(35) TGT	245	20901	(48) TCC	110	2733	(61) CTC	10	4500
(36) TCA	238	23485	(49) CCA	80	22765	(62) ACC	0	3170
(37) GCC	235	12890	(50) TGC	75	4411	(63) AAC	0	2925
(38) TGA	230	24010	(51) TTC	73	1854	(64) AAA	0	29730
(39) TTT	225	16500	(52) AGC	65	6704	Total: 1,261,301