Proteases from Canavalia ensiformis: Active and Thermostable Enzymes with Potential of Application in Biotechnology

Table 2

Proteolytic activity of C. ensiformis extracts on protein substrates.

Extract	Proteolytic activity (U/mg)
	Hemoglobin	Casein	BSA
CE-A	7.3 ± 0.9	7.0 ± 0.4	6.2 ± 0.1
CE-D	9.3 ± 1.6	9.7 ± 0.6	8.4 ± 1.1
CE-P	0.0 ± 0.0	5.6 ± 0.9	1.0 ± 0.7
CE-T	0.0 ± 0.0	5.9 ± 0.3	2.1 ± 0.4
CE-CA	6.8 ± 0.6	6.4 ± 0.6	7.9 ± 1.1
CE-RA	4.0 ± 0.8	3.7 ± 0.8	3.3 ± 1.1
CE-SA	8.1 ± 0.0	1.6 ± 1.7	4.5 ± 1.2

The extracts were incubated with BSA, casein, or hemoglobin for 30 min, reactions were stopped, and absorbance of supernatants was measured at 280 nm. One unit of enzymatic activity (U) refers to the amount of enzyme required to cause an absorbance increase of 0.1 under standard conditions and the specific activity was defined as U·min⁻¹·mg⁻¹ protein.