The Use of Adenovirus Dodecahedron in the Delivery of an Enzymatic Activity in the Cell

Figure 4

Linkage of Pt-Dd and HRP, analyzed by SDS-PAGE and native gel electrophoresis. (a) Coomassie Blue Staining of SDS-PAGE. Purified Pt-Dds after being dialyzed against HEPES buffer 20 mM NaCl 150 mM (A), purified Pt-Dds after being dialyzed against carbonate buffer pH 9.2 overnight at 4°C (B), Pt-Dd linked HRP (C), Bs-Dd linked HRP (D), and High-Range Rainbow Molecular Weight Markers RPN 756E (M). (b) Linkage of Bs-Dd and Pt-Dd to HRP was confirmed by Coomassie Blue Staining of Agarose gel. (c) Linkage of Bs-Dd and Pt-Dd to HRP was confirmed by DAB staining of Agarose gel.

(a)

(b)

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