The Use of Adenovirus Dodecahedron in the Delivery of an Enzymatic Activity in the Cell

Figure 7

Measurement of intracellular peroxidase level of either Pt-Dd-HRP or Bs-Dd-HRP vectors or controls (not coupled with Pt-Dd or free peroxidase). Peroxidase activity is detected by addition of OPD in the soluble cell extract. Peroxidase activity was assessed after 1 h incubation of HeLa cell at 37°C with different concentration (a). Peroxidase activity was assessed over various times of incubation of HeLa cell at 37°C (b). Quantification of internalized vector (c) and attached vector to cell membrane (d) at various times of incubation after HeLa cell was incubated with Pt-Dd-Px for 15 min at 37°C. Error bar represents SEM (). The bars of ELISA assays in these figures represent variation ranges of triplicate measurements.

(a)

(b)

(c)

(d)