Long Noncoding RNA SOX2-OT Exacerbates Hypoxia-Induced Cardiomyocytes Injury by Regulating miR-27a-3p/TGFβR1 Axis
SOX2-OT directly interacts with miR-27a-3p in hypoxic HCMs. (a) The binding site of miR-27a-3p on SOX2-OT was predicted by DIANA tools. (b, e) The overexpression and knockdown efficacy of miR-27a-3p or SOX2-OT was conducted in RT-qPCR. vs. miR-NC. (c) The binding capacity between SOX2-OT and miR-27a-3p was evaluated by using 4 mutations of SOX2-OT in luciferase reporter assay. vs. miR-NC. (d, f) RT-qPCR validated that SOX2-OT and miR-27a-3p negatively regulated each other. vs. miR-NC or NC. (g) RIP assay was adopted to confirm the combination between SOX2-OT and miR-27a-3p. vs. IgG. (h) The level of miR-27a-3p in HCM cells was measured by RT-qPCR. vs. control.
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