Long Noncoding RNA SOX2-OT Exacerbates Hypoxia-Induced Cardiomyocytes Injury by Regulating miR-27a-3p/TGFβR1 Axis
SOX2-OT regulates TGFβR1 level by collaborating with miR-27a-3p in hypoxic HCMs. (a) The binding sequences between miR-27a-3p and TGFBR1 were hypothesized by Targetscan. (b, d) The luciferase activity of wild-type or mutant pGLO-TGFBR1-3′UTR was measured by luciferase reporter assay in HCMs. vs. miR-NC or NC. vs. sh-SOX2-OT. (c, e) The mRNA level of TGFBR1 was monitored by RT-qPCR assay. vs. miR-NC or NC. vs. sh-SOX2-OT. (f, g) The detection of TGFBR1 protein level was carried out in western blot assay. vs. Mock/NC and vs. Double KD.
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