Long Noncoding RNA TUG1 Promotes the Function in ox-LDL-Treated HA-VSMCs via miR-141-3p/ROR2 Axis

Figure 4

ROR2 was a target gene of miR-141-3p. (a) ROR2 was predicted by starBase as a potential target for miR-141-3p. (b) Luciferase reporter assay was conducted to verify the interaction between miR-141-3p and ROR2. (c) The expression of miR-141-3p after the loss-and-gain experiment was measured in ox-LDL-administrated HA-VSMCs using qRT-PCR. (d) The expression of ROR2 after transfection with anti-miR-141-3p or miR-141-3p was detected using western blot. . (e, f) The relative expression of ROR2 was analyzed by qRT-PCR (e) or western blot (f) in tissues of AS patients and healthy participants. (g) The interaction between the expression of ROR2 and miR-141-3p was assayed by qRT-PCR. (h, i) The expression of ROR2 at mRNA (h) and protein (i) in HA-VSMCs treated with ox-LDL increased dose (0 μg/mL, 25 μg/mL, 50 μg/mL, and 75 μg/mL) for 24 h was tested. .

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