Research Article

Knockdown of lncRNA XIST Ameliorates IL-1β-Induced Apoptosis of HUVECs and Change of Tissue Factor Level via miR-103a-3p/HMGB1 Axis in Deep Venous Thrombosis by Regulating the ROS/NF-κB Signaling Pathway

Figure 1

Knockdown of lncRNA XIST attenuated the apoptosis of HUVECs and resulted in a decrease of IL-1β-induced TF level in vivo. (a) The expression levels of lncRNA XIST and miR-103a-3p were detected by qRT-PCR, and the expression level of HMGB1 was analyzed by qRT-PCR and western blot. (b) The correlations among the expression levels of lncRNA XIST, miR-103a-3p, and HMGB1 were calculated by Spearman’s rank-order correlation test. (c) The cell viability of HUVECs under IL-1β addition was determined by MTT assay. (d) The expression levels of lncRNA XIST, miR-103a-3p, and HMGB1 were measured under IL-1β treatment by qRT-PCR. (e) The expression level of lncRNA XIST in transfected HUVECs was analyzed by qRT-PCR, and the cell viability in transfected cells was detected by MTT assay. (f) The cell apoptosis was analyzed by Annexin V-FITC/PI double staining kit. (g) The expression levels of proteins related to apoptosis were detected by western blot. (h) The concentration of TF antigen was determined by the ELISA kit. The nontransfected HUVECs served as the blank group. and .
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