MIR22HG Aggravates Oxygen-Glucose Deprivation and Reoxygenation-Induced Cardiomyocyte Injury through the miR-9-3p/SH2B3 Axis

Figure 3

MIR22HG interacts with miR-9-3p to regulate SH2B3 expression. (a) The possible miRNAs that possibly bind to MIR22HG predicted in starBase. (b) The expression level of miR-9-3p and miR-298-5p in OGD/R-treated HL-1 cells was determined by RT-qPCR (). (c) The overexpression efficiency of miR-9-3p was evaluated by RT-qPCR (). (d and e) The binding sequence between miR-9-3p and MIR22HG is shown. Luciferase reporter assay was used to confirm the binding capacity between miR-9-3p and MIR22HG (). (f) The expression level of candidate target mRNAs in OGD/R-treated HL-1 cells was determined by RT-qPCR (). (g) The binding sequence between miR-9-3p and SH2B3 3 UTR. (h) Luciferase reporter assay was used to validate the binding between miR-9-3p and SH2B3 3 UTR (). (i and j) The mRNA and protein levels of SH2B3 in the context of miR-9-3p overexpression or MIR22HG knockdown were measured by RT-qPCR and western blotting analyses (). , , .

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