Research Article
MIR22HG Aggravates Oxygen-Glucose Deprivation and Reoxygenation-Induced Cardiomyocyte Injury through the miR-9-3p/SH2B3 Axis
Figure 4
Overexpression of SH2B3 reverses the effect of MIR22HG knockdown in OGD/R-treated HL-1 cells. (a) The overexpression efficiency of pcDNA3.1/SH2B3 was tested by RT-qPCR (). (b) The protein level of SH2B3 in each group was measured by western blotting (). (c) The cell viability in different groups was detected by CCK-8 assay (). (d) Cell apoptosis in different groups was assessed by flow cytometry (). (e and f) The protein levels of Bax, Bcl-2, collagen I, fibronectin, and collagen III in OGD/R-treated HL-1 cells transfected with different vectors were measured by western blotting (). , , .
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