Changes in Activity and Kinetic Properties of the Proteasome in Different Rat Organs during Development and Maturation

Figure 1

Michaelis-Menten constants (𝐾_𝑚) of the proteasome in rat liver with age: regression plots of Michaelis-Menten constants (𝐾_𝑚) of the proteasome in rat liver versus age. 𝐾_𝑚-constants were obtained from Lineweaver-Burk plots generated from proteolytic runs using four different substrate concentrations. For each concentration, triplicate samples were run. (a) Chymotrypsin-like activity: 𝑅² of the regression line is 0.049, 𝑃=.138. (b) Trypsin-like activity: 𝑅²=0.417, 𝑃<.001. (c) Peptidylglutamyl peptidase activity: 𝑅²=0.034, 𝑃=.219. A 𝑃-value of less than  .05 was considered statistically significant.

(a) Chymotrypsin-like activity (LLVY)

(b) Trypsin-like activity (VGR)

(c) Peptidylglutamyl-peptidase activity (LLE)