Focal Adhesion-Chromatin Linkage Controls Tumor Cell Resistance to Radio- and Chemotherapy

Figure 1

Cell morphology, HP1α-EGFP distribution and clonogenic radiation survival of cells grown under three-dimensional (3D) growth conditions. (a) Comparison of cell morphology under 2D and 3D growth conditions (green; DAPI, blue, F-actin, red). (b) Fluorescence images of 2D and 3D grown A549 cells expressing HP1α-EGFP fusion protein. Images were acquired using laser scanning microscopy. (c) 2D and 3D clonogenic radiation survival of HP1α-EGFP expressing A549 cells irradiated with single doses of X-rays (0–6 Gy). Means ± SD and Student’s 𝑡-test comparing 3D versus 2D conditions. *𝑃<0.05; **𝑃<0.01; 𝑛=3; bar, 10 μm.

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