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Cholesterol
Volume 2015, Article ID 147601, 3 pages
http://dx.doi.org/10.1155/2015/147601
Research Article

Nonselective Mevalonate Kinase Inhibitor as a Novel Class of Antibacterial Agents

1Department of Medicine, Islamic Azad University, Qom Branch, Qom 37157, Iran
2Department of Biochemistry, Payam Nour University of Mashhad, Mashhad 37157, Iran
3Department of Biochemistry, School of Medicine, Qom University of Medical Science, Qom 37157, Iran
4Booali Medical Research Center, Qom 37157, Iran

Received 30 September 2014; Revised 8 December 2014; Accepted 1 January 2015

Academic Editor: Shinichi Oikawa

Copyright © 2015 Mohammad Gharehbeglou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. There are a few evidences about targeting isoprenoids biosynthesis pathway in bacteria for finding new antibiotics. This study was conducted to assess antibacterial effects of vanadyl sulfate (VS), one of the mevalonate kinase inhibitors to find a new target for killing bacteria. Materials and Methods. Antibacterial effect of VS alone and in combination with glycine or EDTA was assessed on Escherichia coli and Pseudomonas aeruginosa as Gram-negative and Staphylococcus aureus and Enterococcus faecalis as Gram-positive bacteria using serial dilution method and minimum inhibitory concentrations (MICs) identified. Result. MICs for S. aureus and E. coli were 4 and 8 mg/mL, respectively. VS could not affect the growth of two other bacteria. However, VS in combination with glycine not only inhibited the growth of E. faecalis and P. aeruginosa, but also reduced MICs for VS-sensitive bacteria (S. aureus and E. coli). EDTA could reduce MIC for E. coli and P. aeruginosa. Conclusion. VS could inhibit the growth of S. aurous and E. coli, and adding glycine or EDTA improved VS antibacterial activity presumably via instability of the cell wall and enhanced transport of VS through bacterial cell wall. Inhibition of the isoprenoid pathway might provide new tools to overcome bacterial resistance.