Figure 2: KNEWRiTE, our new software for segmentation of single neuron morphology. (a) Screenshot of the software. The software can trace dendritic and axonal trees automatically, and allows manual editing and addition of segments that are difficult to detect automatically. (b) Structure of KNEWRiTE. The “Stack Image Loader” and “Cell Generator” load LSM image data and separate neurons from background by binarization. The neuron structure is extracted by “Extraction Tools,” an automatic extractor and data editor. The results are exported as image data and a morphological model in SWC format.