Figure 6: Differences in coordinate values of distinct points before and after registration. (a) Twelve landmarks for registration in the standard brain (cross) were assigned from the edges of three clearly delineated regions, the central body and themushroom body calyces. Six distinct points (circles), the center of the central body (CCB), the centers of the calyces (RMC and LMC), and the peduncles (RMP and LMP) of mushroom bodies were selected to calculate the registration error. (b) Euclidian distances were measured between distinct points in the standard brain and each sample brain before and after registration. The average and standard deviation are plotted for each point. With the exception of LMP, there were significant differences based on the one-way ANOVA before and after registration.