Table of Contents
Chinese Journal of Biology
Volume 2014, Article ID 208462, 14 pages
http://dx.doi.org/10.1155/2014/208462
Research Article

Evaluation of Production Parameters for Maximum Lipase Production by P. stutzeri MTCC 5618 and Scale-Up in Bioreactor

Department of Microbial Biotechnology, Panjab University, Chandigarh 160014, India

Received 22 July 2014; Revised 17 September 2014; Accepted 23 September 2014; Published 30 October 2014

Academic Editor: Lida Zhang

Copyright © 2014 Vishal Thakur et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Intracellular lipase producer screened from the library available in the laboratory, identified through 16S rRNA as Pseudomonas stutzeri, was studied for maximum enzyme production in shake flask. The work was intended to evaluate the effect of different physicochemical factors like carbon, nitrogen, metal ions, surfactant, inoculum, pH, temperature, agitation, and aeration on lipase production. Optimized media showed 1.62-fold increase in lipase production when compared to basal media. Scale-up of lipase in in situ bioreactor showed reduction in fermentation time in both basal and optimized media, giving 41 and 99 U/mg of lipase activity after 48 h of fermentation.