Hepatoprotective Effect of Citrus limon Fruit Extract against Carbofuran Induced Toxicity in Wistar Rats

Figure 10

Effect of CF on the level of liver total thiol and amelioration by C. limon fruit extract. Control: control group treated with 0.5 mL groundnut oil; 20% CF: three subacute oral doses (1.6 mg Kg⁻¹ bwt equivalent to 20% LD₅₀) of CF. C. limon: the rats treated with 0.5 mL C. limon fruit extract three times at the intervals of 24 h. C. limon + 20% CF refer to the experimental animals group which received 0.5 mL C. limon fruit extract 30 min prior to the treatment of rats with CF (1.6 mg Kg⁻¹ bwt). All the treatments were given as three-time oral doses at the interval of 24 h and experiments were carried out up to three days. The animals were sacrificed after 24 h from the last dose of treatment. Level of liver total thiol was determined as described in Materials and Methods. The unit of level of total thiol is μg mg⁻¹ protein. The values were expressed as Mean ± SD; , where is number of determinations. The signs and indicate values significantly different from control at and , respectively.