Table of Contents
Chinese Journal of Biology
Volume 2016 (2016), Article ID 6420371, 7 pages
http://dx.doi.org/10.1155/2016/6420371
Research Article

HPTLC Fingerprint Profile (Phenolics) of Selected Cyathea Species from Western Ghats, South India

Centre for Plant Biotechnology, Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tamil Nadu 627 002, India

Received 25 August 2015; Revised 2 January 2016; Accepted 4 January 2016

Academic Editor: Vivian Y. Shin

Copyright © 2016 Janakiraman Narayanan and Johnson Marimuthu alias Antonysamy. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

HPTLC analysis was performed to study the phenolics, flavonoids, and tannins profile of Cyathea nilgirensis Holttum, Cyathea gigantea (Wall. ex. Hook.) Holttum, and Cyathea crinita (Hook.) Copel. The maximum number of bands was observed in the ethanolic extracts of C. crinita. The phenolic bands with values 0.05, 0.32, 0.43, 0.75 (quercetin), and 0.83 demonstrated their occurrence in all the three studied species of Cyathea. Flavonoids with values 0.05, 0.23, 0.30, 0.43, and 0.73 showed their presence in the three studied species of Cyathea. Tannins with the values 0.05, 0.09, and 0.82 determined their existence in all the three studied Cyathea species. Gallic acid was present only in C. crinita with the value 0.49. The developed HPTLC profiles can be used for identification and quantitative determination of phenolics, flavonoids, and tannins. This method is also suitable for rapid screening of Cyathea species for chemotypic assessment and also for quality control purposes.