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Canadian Journal of Gastroenterology
Volume 25, Issue 6, Pages 327-329
http://dx.doi.org/10.1155/2011/758579
Original Article

Antibodies from a Patient with Type 1 Diabetes and Celiac Disease Bind to Macrophages that Express the Scavenger Receptor CD163

Brigitte Sonier,1,2 Alexander Strom,1 Gen-Sheng Wang,1 Christopher Patrick,1,2 Jennifer A Crookshank,1 Majid Mojibian,1,2 Amanda J MacFarlane,1,2 and Fraser W Scott1,2,3

1Chronic Disease Program, Ottawa Hospital Research Institute, Canada
2Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada
3Department of Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada

Received 15 October 2010; Accepted 15 December 2010

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Antibodies against the wheat storage globulin Glo-3A from a patient with both type 1 diabetes (T1D) and celiac disease were enriched to identify potential molecular mimicry between wheat antigens and T1D target tissues. Recombinant Glo-3A was used to enrich anti-Glo-3A immunoglobulin G antibodies from plasma by batch affinity chromatography. Rat jejunum and pancreas, as well as human duodenum and monocytes were probed, and binding was evaluated by immunohistochemistry and confocal microscopy. Glo-3A-enriched antibodies bound to a specific subset of cells in the lamina propria of rat jejunum that co-localized mostly with a marker of resident, alternatively activated CD163-positive (CD163+) macrophages. Blood monocytes and macrophage-like cells in human duodenum were also labelled with the enriched antibodies. Blocking studies revealed that binding to CD163+ macrophages was not due to cross-reactivity with anti-Glo-3A antibodies, but rather to non-Glo-3A antibodies co-purified during antibody enrichment. The novel finding of putative autoantibodies against tolerogenic intestinal CD163+ macrophages suggests that regulatory macrophages were targeted in this patient with celiac disease and T1D.