Possible Involvement of Liver Resident Macrophages (Kupffer Cells) in the Pathogenesis of Both Intrahepatic and Extrahepatic Inflammation

Figure 1

Treatment with clodronate liposome deletes Kupffer cells but does not affect other cell populations. DBA/2 mice were intravenously administered with 100 ul of clodronate liposome. (a) Nonparenchymal cells from the livers were collected by collagenase infusion described in Material and Methods at indicated days after treatment and then subjected to flow cytometry analysis using anti-F4/80 and anti-CD11b antibodies. The population of Kupffer cells was indicated as F4/80 positive and CD11b middle positive population. (b) Splenocytes from control or treated mice 3 days after treatment were collected and subjected to flow cytometry analysis for helper T cells (CD3⁺CD4⁺), dendritic cells (DC) (CD3⁻CD11b⁺), and regulatory T cells (CD4⁺Foxp3⁺). A, B, D, and E are target populations.

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