Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages

Figure 4

Proliferation of microencapsulated SGC9701 cells by BrdU staining. Brown or yellow nuclear staining indicates live cells. After culturing for (a) 7 days or (b) 14 days, the cells tended to aggregate into spheres. The entire microencapsulated spheres showed positive BrdU staining. (c) After 21 days, the staining color faded or the center of the cells showed no staining while the cells outside still showed staining.

(a)

(b)

(c)