Loss of LLGL1 Expression Correlates with Diffuse Gastric Cancer and Distant Peritoneal Metastases

Figure 2

(a) RT-PCR and Western Blot confirms successful transfection of SNU16 cancer cells with LLGL1-GFP. (b) Fluorescence microscopy confirms the membranous accumulation of GFP-LLGL1 fusion protein in comparison to the cytoplasmic GFP localization of GFP only expressing cells. (c) LLGL1 reexpression in SNU16 did not impact on proliferation, apoptosis, or invasion. However, reexpression of LLGL1 in SNU16 resulted in a significant increase of adhesion to plastic and extracellular matrix proteins laminin and fibronectin.

(a)

(b)

(c)