Ischemic Postconditioning (IPostC) Protects Fibrotic and Cirrhotic Rat Livers after Warm Ischemia
Lactate dehydrogenase, bile flow, and portal perfusion pressure in healthy, fibrotic, and cirrhotic livers after warm ischemia ± IPostC. Liver fibrosis was induced in male SD rats using bile duct ligation (BDL) over 4 weeks. Liver cirrhosis was induced using thioacetamide (TAA) administered via drinking water over 18 weeks. The healthy, fibrotic, and cirrhotic livers were perfused for 30 min, followed by 90 min of ischemia at 37°C and 90 min of reperfusion. The IPostC intervention groups included 8x20 sec and 4x60 sec cycles that were performed after ischemia (control and intervention groups, each n=8). Lactate dehydrogenase (LDH) was measured in the perfusate and is represented as total value (a) and during the early phase (minute 0 to 40; (b)) and the late phase (minute 50 to 90; (c)) of reperfusion. Bile was collected and also the bile flow is shown as total value (d) and at the early (e) and late (f) phase of reperfusion. For the portal perfusion pressure, which was monitored continuously, the maximum (g) and the value at the end of perfusion (h) are displayed. All data are expressed as the mean ± SEM, and significant values are specified as p<0.05 and p<0.01.
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