Ischemic Postconditioning (IPostC) Protects Fibrotic and Cirrhotic Rat Livers after Warm Ischemia
Thromboxane B2 in healthy, fibrotic, and cirrhotic livers after warm ischemia ± IPostC. The induction of liver fibrosis using bile duct ligation (BDL) over 4 weeks or the induction of liver cirrhosis using thioacetamide (TAA) administered via drinking water over 18 weeks in male SD rats was performed. The healthy, fibrotic, and cirrhotic livers were perfused for 30 min and stored at 37°C for 90 min, followed by reperfusion for 90 min. After ischemia, IPostC interventions using 8x20 sec and 4x60 sec (control and intervention groups, each with n=8) were carried out. Thromboxane B2 (TXB2) was measured in the collected perfusate using an enzyme-linked immunosorbent assay. The values for TXB2 are shown as basal values (a), in total (b) and during the early phase (minute 0 to 40; (c)) and the late phase (minute 50 to 90; (d)) of reperfusion. All data are expressed as the mean ± SEM. Significant values are specified as p<0.05 and p<0.01.
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