A New CYP2E1 Inhibitor, 12-Imidazolyl-1-dodecanol, Represents a Potential Treatment for Hepatocellular Carcinoma

Figure 2

mRNA-expression levels of genes related to apoptosis and cell-cycle progression in HepG2 cells. (a, b) Cutoff values of ≥+5 and ≤−7.5 were determined, which resulted in the selection of 21 out of 88 analysed genes. These most dysregulated genes associated with cell-cycle progression and apoptosis were selected for further validation. BIRC5 expression was included in both arrays. Genes whose relative mRNA-expression levels could not be evaluated (due to no detectable amplification) were excluded from the analysis. (c) The relative mRNA-expression levels of the selected 21 genes were analysed in three independent experiments performed with HepG2 cells treated with I-ol (100 μM) for 48 h. The expression level of each gene was ranked, where the highest expression levels corresponded with the highest rank. Finally, the sums of all three ranking values were calculated for each gene and are depicted as stacked columns. In concrete terms, this ranking indicated that TNFSF11 was the least expressed gene and that TNFSF15 the most expressed gene. Three differentially expressed genes (TNFSF15, CDKN1B, and CDKN2B) showed repressed mRNA expression compared to the control group treated with 100 μM CD.

(a)

(b)

(c)