A New CYP2E1 Inhibitor, 12-Imidazolyl-1-dodecanol, Represents a Potential Treatment for Hepatocellular Carcinoma

Figure 6

DLD-1 cell growth into CAM tissues. (a) Representative images of H&E staining, Ki-67 and p27 immunohistochemistry, and TUNEL assay results. The magnification of the objective lens was 20-fold, and that of the ocular lens was 10-fold, such that the total magnification was 200-fold. (b) A gradual reduction of Ki-67-positive cells was observed with increasing I-ol concentrations. (c) Induction of p27 protein expression. (d) Linear increase in the number of apoptotic cells, compared with the control groups. The results are shown as the mean ± SD. Statistical calculations were performed based on two-way mixed ANOVA, followed by Bonferroni’s post hoc test. In all cases, the significance level was (significant), (highly significant), or (very highly significant). Dose-dependent effects of I-ol were calculated by determining Pearson’s correlation coefficient (r) and performing linear-regression analysis (adjusted R²), with the following statistical parameters: (significant), (highly significant), and (very highly significant).

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