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Canadian Journal of Infectious Diseases
Volume 5, Issue 4, Pages 170-178
http://dx.doi.org/10.1155/1994/856850
Original Article

Passive Protection of Diabetic Rats with Antisera Specific for the Polysaccharide Portion of the Lipopolysaccharide Isolated from Pseudomonas pseudomallei

Larry E Bryan,1 Sallene Wong,1 Don E Woods,1 David AB Dance,2 and W Chaowagul3

1Department of Microbiology and Infectious Diseases, University of Calgary and Foothills Hospital, Calgary, Alberta, Canada
2Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, University of London, London, UK
3Department of Medicine, Sapprasitprasong Hospital, Ubon Ratchatani, Thailand

Received 22 October 1993; Accepted 20 January 1994

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Polyclonal and monoclonal antisera raised to tetanus toxoid-conjugated polysaccharide of lipopolysaccharide (lps) and purified lps of Pseudomonas pseudomallei that reacted with a collection of 41 strains of this bacterium from 23 patients are described. The common antigen recognized by these sera was within the polysaccharide component of the lps of the cells. The sera were specific for P pseudomallei in that none of 37 strains of other bacteria, including 20 Gram-negative and three Gram-positive species, were recognized, although cross-reaction occurred using the anticonjugate serum with some strains of Pseudomonas cepacia serotype A, a closely related bacterium. Passive protection studies using a diabetic rat model of P pseudomallei infection showed that partially purified rabbit polyclonal and mouse monoclonal antisera were protective when the median lethal dose was raised by four to five orders of magnitude. The wide distribution of the polysaccharide antigen among isolates of P pseudomallei used in this study and the protective role of antibody to the conjugated polysaccharide antigen suggest potential as a vaccine.