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Canadian Journal of Infectious Diseases
Volume 8, Issue 6, Pages 318-322
http://dx.doi.org/10.1155/1997/828612
Original Article

Epidemiological Investigation of Salmonella Tilene by Pulsed-Field Gel Electrophoresis and Polymerase Chain Reaction

Chandar M Anand,1 Kevin Fonseca,1,3 Ken Longmore,2 Robert Rennie, Linda Chui,1,3 Mike Lingley,1 and David Woodward4

1Provincial Laboratory of Public Health for Southern Alberta, Calgary, Alberta, Canada
2Palliser Health Authority, Environmental Health Services, Medicine Hat, Alberta, Canada
3Department of Microbiology and Public Health, University of Alberta Hospital, Edmonton, Alberta, Canada
4National Reference Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada

Received 4 December 1996; Accepted 12 March 1997

Copyright © 1997 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pulsed-field gel electrophoresis (PFGE) and DNA fingerprinting by the polymerase chain reaction (PCR) were performed on 11 isolates of Salmonella tilene. Five strains were from a cluster of human patients, six from sugar gliders and pygmy hedgehogs kept as family pets or from local pet retailers, and one isolate from the first North American case of S tilene described in Washington State in 1994. The PFGE restriction patterns showed all isolates to be similar. However, PCR using primers to the 16S and 23S rRNA genes of Escherichia coli demonstrated that the Washington State isolate differed from the rest of the other isolates, which were all similar based upon their DNA fingerprint. This study indicates that reliance on one technique alone may be insufficient to show nuances between strains that are, in many respects, closely related.