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Canadian Journal of Infectious Diseases and Medical Microbiology
Volume 2016 (2016), Article ID 3076380, 6 pages
http://dx.doi.org/10.1155/2016/3076380
Clinical Study

Human Papilloma Virus Persistence after Cone Excision in Women with Cervical High Grade Squamous Intraepithelial Lesion: A Prospective Study

1Department of Obstetrics and Gynecology, University of Medicine and Pharmacy “Victor Babeş”, Timişoara, 300041 Timiș, Romania
2Department of Anatomy, University of Medicine and Pharmacy “Victor Babeş”, Timişoara, 300041 Timiș, Romania
3Department of Obstetrics and Gynecology, County Hospital Timişoara, 300202 Timiș, Romania
4Department of Informatics and Statistics, University of Medicine and Pharmacy “Victor Babeş”, Timişoara, 300041 Timiș, Romania
5Department of Surgery, University of Medicine and Pharmacy “Victor Babeş”, Timişoara, 300041 Timiș, Romania
6Department of Microbiology, University of Medicine and Pharmacy “Victor Babeş”, Timişoara, Piata Eftimie Murgu, No. 1, 300041 Timiș, Romania

Received 13 February 2016; Revised 14 April 2016; Accepted 20 April 2016

Academic Editor: Bruno Pozzetto

Copyright © 2016 Laurențiu Pirtea et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Persistent human papillomavirus (HPV) infection is a necessary event in cervical cancer tumorigenesis. Our objectives were to estimate the rate of HPV infection persistence after large loop excision of the transformation zone (LEEP) in patients with high grade squamous intraepithelial lesions (HSIL) and to investigate if HPV persistence is type related. Methods. We conducted a prospective study on 89 patients with HSIL treated with LEEP. DNA HPV was performed before surgery and at 6, 12, and 18 months after LEEP. Results. Four patients were excluded from the study. The HPV persistence in the remaining 85 patients was 32.95% (6 months), 14.12% (12 months), and 10.59% (18 months). Type 16 had the highest persistence rate, 23.5% (6 months), 11.8% (12 months), and 8.2% (18 months). Coinfection was found to be 54.12% before LEEP and 18.8% (6 months), 4.7% (12 months), and 3.5% (18 months) after LEEP. The rate of coinfections including type 16 was 46.83% of all coinfections. Coinfection including type 16 was not correlated with higher persistence rate compared to infection with type 16 only. Conclusions. HPV infection is not completely eradicated by LEEP in patients with HSIL lesion on PAP smear. HPV persistence after LEEP is influenced by HPV type. HPV type 16 has the highest persistence rate.