Evidences of the Low Implication of Mosquitoes in the Transmission of Mycobacterium ulcerans, the Causative Agent of Buruli Ulcer
Table 4
MU distribution among mosquitoes developmental stages, cuticles, and breeding waters.
Nature of the samples
Mosquitoes developmental stages
Distribution of MU targets
Pool positive/pool tested
Presence of MU
Mean Cts (IS2404)
Mean Cts (KR-B)
Eggs
19 ± 1.79
21 ± 2.22
4/4
Yes
L1
27.67 ± 2.66
31.59 ± 3.15
4/4
Yes
L2
29.92 ± 2.58
33.06 ± 2.98
4/4
Yes
L3
31.36 ± 2.98
34.33 ± 3.34
4/4
Yes
L4
31.38 ± 2.20
35.03 ± 1.17
3/4
Yes
Pupae
NoCt
NoCt
0/4
No
Adults
37.89
NoCt
0/4
No
Mosquito
Eggs
NA
NA
NA
NA
L1
30.72 ± 1.78
36.51 ± 2.09
4/4
Yes
L2
34.25 ± 2.83
36.82 ± 1.65
3/4
Yes
L3
34.13
39.53
1/4
Yes
L4
NoCt
NoCt
0/4
No
Pupae
38
NoCt
0/4
No
Adults
NA
NA
NA
NA
Mosquito breeding
Eggs
18.43 ± 2.03
21.49 ± 1.63
4/4
Yes
L1
23.04 ± 3.19
30.61 ± 2.80
4/4
Yes
L2
22.71 ± 2.59
31.88 ± 2.60
4/4
Yes
L3
28.4 ± 2.86
33.53 ± 3.00
4/4
Yes
L4
32.00 ± 2.64
35.94 ± 1.04
3/4
Yes
Pupae
33.65
35.47
1/4
Yes
Adults
NA
NA
NA
NA
L1, 2, 3, and 4 correspond to first, second, third, and fourth instars larvae, respectively. Yes or no corresponds to the presence or the absence of the bacteria in analyzed samples. NA stands for not applicable. bacterial loads did not vary significantly among the developmental stages ().
