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Canadian Journal of Infectious Diseases and Medical Microbiology
Volume 2017, Article ID 2191532, 6 pages
https://doi.org/10.1155/2017/2191532
Research Article

Detection of Methicillin Resistant Staphylococcus aureus and Determination of Minimum Inhibitory Concentration of Vancomycin for Staphylococcus aureus Isolated from Pus/Wound Swab Samples of the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal

1Department of Microbiology, Goldengate International College, Battisputali, Kathmandu, Nepal
2Department of Microbiology, Grande International Hospital, Dhapasi, Kathmandu, Nepal
3Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal
4Department of Microbiology, Nepalese Army Institute of Health Sciences, Sanobharyang, Kathmandu, Nepal

Correspondence should be addressed to Narayan Dutt Pant; moc.liamg@7891tnapdn

Received 9 September 2016; Accepted 14 December 2016; Published 5 January 2017

Academic Editor: Jorge Garbino

Copyright © 2017 Raghabendra Adhikari et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study was conducted to evaluate the performance of cefoxitin disc diffusion method and oxacillin broth microdilution method for detection of methicillin resistant S. aureus (MRSA), taking presence of mecA gene as reference. In addition, inducible clindamycin resistance and beta-lactamase production were studied and minimum inhibitory concentration (MIC) of vancomycin for S. aureus isolates was determined. A total of 711 nonrepeated pus/wound swab samples from different anatomic locations were included in the study. The Staphylococcus aureus was identified on the basis of colony morphology, Gram’s stain, and biochemical tests. A total of 110 (15.47%) S. aureus isolates were recovered, of which 39 (35.50%) isolates were identified as MRSA by cefoxitin disc diffusion method. By oxacillin broth microdilution method, 31.82% of the Staphylococcus aureus isolates were found to be MRSA. However, mecA gene was present in only 29.1% of the isolates. Further, beta-lactamase production was observed in 71.82% of the isolates, while inducible clindamycin resistance was found in 10% of S. aureus isolates. The MIC value of vancomycin for S. aureus ranged from 0.016 μg/mL to 1 μg/mL. On the basis of the absolute sensitivity (100%), both phenotypic methods could be employed for routine diagnosis of MRSA in clinical microbiology laboratory; however cefoxitin disc diffusion could be preferred over MIC method considering time and labour factor.