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Canadian Journal of Infectious Diseases and Medical Microbiology
Volume 2017 (2017), Article ID 7058396, 5 pages
https://doi.org/10.1155/2017/7058396
Research Article

Colistin Resistance of Pseudomonas aeruginosa Isolated from Snakes in Taiwan

1Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan
2Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan
3Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan
4Veterinary Teaching Hospital, College of Veterinary Medicine, National Chiayi University, Chiayi City, Taiwan
5Laboratory Animal Service Center, Office of Research and Development, China Medical University, Taichung 40402, Taiwan
6Department of Emergency Medicine, Taichung Veterans General Hospital, Taichung 40402, Taiwan

Correspondence should be addressed to Kwong-Chung Tung; moc.liamg@89gnutck

Received 9 May 2017; Accepted 17 August 2017; Published 25 September 2017

Academic Editor: Branka Bedenić

Copyright © 2017 Po-Yu Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study included fifty-eight isolates of P. aeruginosa from the oral cavity of snakes that were recruited from clinical cases, captive and wild snakes. The minimum inhibitory concentrations (MICs) for the determination of susceptibility were identified by the broth microdilution method. Polymerase chain reaction (PCR) was employed to detect β-lactamases genes. With regard to antipseudomonal antibiotics, the lowest nonsusceptible rates were in aztreonam (15%), piperacillin/tazobactam (12%), and amikacin (9%). The nonsusceptible rates were high in gentamicin (33%) and colistin (55%). Meanwhile, presented in 100% of isolates where , , and came at 94.8%, 89.7%, and 27.6%, respectively. Emergence of multidrug resistant (MDR) strains and colistin-resistant strains highlights the potential breach of public health as P. aeruginosa could be transmitted through either direct contact or indirect dissemination through the environment. This study reports that the highly resistant P. aeruginosa from snakes’ oral cavity were discovered for the very first time in Taiwan.